Nectin3 Mouse shRNA Plasmid (Locus ID 58998)

Specifications

Product Data
Product Name	Nectin3 Mouse shRNA Plasmid (Locus ID 58998)
Locus ID	58998
UniProt ID	Q9JLB9
Synonyms	2610301B19Rik; 3000002N23Rik; 4921513D19Rik; AA407785; AU016832; AW538082; CD113; Pvrl3
Vector	pRFP-C-RS
Format	Retroviral plasmids
Kit Components	Pvrl3 - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 58998). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq	BC125588, BC132187, NM_021495, NM_021496, NM_021497, NM_021497.1, NM_021497.2, NM_021495.1, NM_021495.2, NM_021495.3, NM_021495.4, NM_021496.1, NM_021496.2, NM_021496.3, BC096530
Summary	Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.