ULK3 Human shRNA Plasmid Kit (Locus ID 25989)

CAT#: TF317070

DKFZP434C131 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided



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CNY 4,790.00


货期*
现货

规格
    • 1 kit

Product images

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Specifications

Product Data
Product Name ULK3 Human shRNA Plasmid Kit (Locus ID 25989)
Locus ID 25989
UniProt ID Q6PHR2
Vector pRFP-C-RS
Format Retroviral plasmids
Kit Components DKFZP434C131 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 25989). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_001099436, NM_001284364, NM_001284365, NM_015518, NR_104307, NR_104308, NR_104309, NM_001099436.1, NM_001099436.2, NM_001099436.3, NM_001284365.1, NM_001284365.2, NM_001284364.1, NM_001284364.2, NM_015518.1, BC036117, BC048289, BC056423, BC157884, NM_001284364.3, NM_001099436.4, NM_001284365.3
Summary Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.[UniProtKB/Swiss-Prot Function]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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