PTP1B (PTPN1) (NM_002827) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding the region (Glu2-Asn321) of Homo sapiens PTPN1.
Tag	N-His
Predicted MW	39.2 KDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM HEPES, pH 7.5, 50 mM NaCl, 2.5 mM EDTA, 5 mM DTT
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Specific activity: ~14000 nmol/min/mg. Enzymatic activity was determined by measuring the amount of 4-Nitrophenol hydrolyzed from p-nitrophenyl phosphate (pNPP) at 30C.
Reference Data
RefSeq	NP_002818
Locus ID	5770
UniProt ID	P18031
Refseq Size	3573
Cytogenetics	20q13.13
Refseq ORF	1305
Synonyms	PTP1B
Summary	The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
Protein Families	Druggable Genome, Phosphatase, Transmembrane
Protein Pathways	Adherens junction, Insulin signaling pathway

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