FGF2 (NM_002006) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding the region (Gly132-Ser288) of human FGF2
Tag	Tag Free
Predicted MW	17.4 kDa
Concentration	>0.1 µg/µL as determined by microplate BCA method
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from a sterile solution containing 10 mM Tris-HCl, pH7.5, 150 mM NaCl，8% trehalose
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -20°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Measured in a cell proliferation assay using 3T3 cells. ED50 for this effect is typically 0.07-0.10 ng/ml. Cell culture factor (PMID: 26253201) Cell culture factor (PMID: 26704449) Cell culture factor (PMID: 29416007) Cell culture factor (PMID: 29937202) Cell culture factor (PMID: 29948854)
Endotoxin	< 0.1 EU per 1 µg of the protein by the LAL
Reference Data
RefSeq	NP_001997
Locus ID	2247
UniProt ID	P09038
Refseq Size	6803
Cytogenetics	4q28.1
Refseq ORF	864
Synonyms	BFGF; FGF-2; FGFB; HBGF-2
Summary	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

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