Recombinant IL-36 alpha (Interleukin-36 alpha)，Mouse

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MNKEKELRAASPSLRHVQDLSSRVWILQNNILTAVPRKEQTVPVTITLLPCQYLDTLETNRGDPTYMGVQRPMSCLFCTKDGEQPVLQLGEGNIMEMYNKKEPVKASLFYHKKSGTTSTFESAAFPGWFIAVCSKGSCPLILTQELGEIFITDFEMIVVH with polyhistidine tag at the C-terminus.
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 18.82 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <15 ng/mL. The specific activity of recombinant mouse IL-36 alpha is > 1 x 10⁵ IU/mg.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	Q9JLA2
Synonyms	IL36A, Interleukin-1 family member 6 (IL-1F6), FIL1ε (FIL1E), Interleukin-1ε (IL1E), Fi, Fil, Fil1, IL-1, IL-1H1, IL1RP2
Summary	Interleukin 36 alpha (IL-36 alpha) is a 17.76 kDa cytokine with 160 amino acid residues. IL-36 alpha is an IL-1 family member, which binds to the Interleukin-1 receptor and is expressed in the lung, stomach, and placenta. IL-36 alpha is critical in inflammatory responses and stimulates the production of proinflammatory cytokines, such as IL-12, IL-1β, IL-6, and TNF-α. It also induces the generation of IFNγ, IL-4, and IL-17 in CD4+ T cells.

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