Recombinant NRG1，Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding Human NRG1 Protein (#Q02297)(Ser20-Lys241) was expressed.
Tag	Tag Free
Predicted MW	The protein has a calculated MW of 24.29 kDa. The protein migrates as 28-30 kDa under reducing condition (SDS-PAGE analysis).
Purity	>95% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Testing in process
Endotoxin	<1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	Q02297
Synonyms	Acetylcholine receptor-inducing activity (ARIA), Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin (HRG), Neu differentiation factor, Sensory and motor neuron-derived factor
Summary	Neuregulin-1 (NRG-1, also called heuregulin1 or neu differentiation factor) is a glycoprotein that belongs to the neuregulins family. Structurally, Neuregulin-1 harbors tissue-specific N terminal sequence, followed by immunoglobulin-like (Ig-like) domains, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. Neuregulin-1 exerts its actions upon engagement of its EGF-like domain to the extracellular binding region of HER RTK family members (EGFR, HER2, HER3, and HER4), subsequently leading to receptor dimerization which activates a variety of signal pathways such as MAPK-ERK and PI3K-AKT pathways. These signal cascades are essential for regulating cardiac development, neuronal differentiation, neuromuscular synapses formation, and stem cell proliferation.

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