Recombinant HGF， Human， HEK293

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding Human HGF Protein (#P14210)(Gln 32-Ser 728) was expressed with polyhistidine tag at the C-terminus.
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 80.48 kDa. The protein migrates as 75-100 kDa under reducing condition (SDS-PAGE analysis).
Purity	>90% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Testing in process
Endotoxin	<1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	P14210
Synonyms	Hepatocyte growth factor, Hepatopoietin-A, Scatter factor (SF), HPTA
Summary	Hepatocyte Growth Factor (HGF) activates c-Met receptor's tyrosine kinase cascade, influencing cell growth and motility. Originating from mesenchymal cells, HGF is a multifunctional cytokine that plays a central role in angiogenesis, tumorigenesis, and tissue regeneration. Initially secreted as an inactive polypeptide, HGF undergoes cleavage to become an active heterodimeric molecule. The gene exhibits alternative splicing, resulting in diverse isoforms.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.