Recombinant Heregulin Beta 1，Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding Human Heregulin Beta 1 Protein (#Q02297-6)(Ser177-Glu241) was expressed with polyhistidine tag at the C-terminus.
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 8.42 kDa. The protein migrates as 8 kDa under reducing condition (SDS-PAGE analysis).
Purity	>95% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is < 10 ng/mL.
Endotoxin	<1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	Q02297-6
Synonyms	NRG1 Beta 1, Heregulin-β1
Summary	Neuregulin-1 (NRG-1, also called heuregulin1 or neu differentiation factor) is a glycoprotein that belongs to the neuregulins family. Structurally, Neuregulin-1 harbors tissue-specific N terminal sequence, followed by immunoglobulin-like (Ig-like) domains, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. NRG1 has multiple isoforms produced by alternative splicing. Heregulin- β1 (HRG- β1) is one of the isoforms, has been reported to engage the development and survival of cardiomyocytes derived from embryonic stem (ES) cells via activating MAPK-ERK and PI3K-AKT pathways. Moreover, HRG- β1 plays a central role in promoting the proliferation of neuronal progenitors from embryonic neural stem cells.

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