Recombinant FGF-4 (Fibroblast growth factor-4)，Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGRGGAAAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL with polyhistidine tag at the C-terminus
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 20.70 kDa. The protein migrates as 22 kDa under reducing condition (SDS-PAGE analysis).
Purity	>95% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 0.1% sarkosyl in 1X PBS, pH 8.0.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2.5 ng/mL. The specific activity of recombinant human FGF-4 is > 4 x 10⁵ IU/mg.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	P08620
Synonyms	HST-1, Transforming protein KS3, HBGF-4
Summary	Fibroblast Growth Factors-4 (FGF-4) is a 22 kDa member of the fibroblast Growth Factors with 206 amino acid residues. FGF-4 can regulate embryonic development, cell proliferation, and cell differentiation. FGF-4 is an important role development during embryogenesis.

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