Recombinant FGF-22 (Fibroblast growth factor-22)，Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) TPSASRGPRSYPHLEGDVRWRRLFSSTHFFLRVDPGGRVQGTRWRHGQDSILEIRSVHVGVVVIKAVSSGFYVAMNRRGRLYGSRLYTVDCRFRERIEENGHNTYASQRWRRRGQPMFLALDRRGGPRPGGRTRRYHLSAHFLPVLVS with polyhistidine tag and SUMO tag at the N-terminus.
Tag	His-SUMO Tag (N-term)
Predicted MW	The protein has a calculated MW of 29.35 kDa. The protein migrates as 36 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 ng/mL.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	Q9HCT0
Synonyms	UNQ2500/PRO5800
Summary	Fibroblast Growth Factors-22 (FGF-22) is a 22.3 kDa member of the fibroblast Growth Factors with 209 amino acid residues. FGF-22 is expressed from brain, skin, horizontal cells, bipolar cells, rod photoreceptor cells, muller glia cells. Involving fasting response, glucose homeostasis, lipolysis, lipogenesis and hair development.

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