Recombinant CNTF (Ciliary neurotrophic factor)，Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM with polyhistidine tag at the C-terminus
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 23.74 kDa. The protein migrates as 25 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to induce proliferation in TF-1 cells. The ED₅₀ for this effect is <0.15 μg/mL.
Endotoxin	<0.01 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	P26441
Synonyms	HCNTF
Summary	Ciliary neurotrophic factor (CNTF) is the member of IL-6 cytokine family and expressed in the nervous system. CNTF is 22.9 kDa neurotrophic factor containing 200 residues, which shows multiple effects in vertebrate retinogenesis. Besides, CNTF acts as a promoter that not only accelerates adult neurogenesis but also increases the survival of neuron after injury. On the other hands, reducing number of hippocampal GABAergic interneurons, as well as 5-HT levels and 5-HT1A receptor expression has been found in CNTF knockout female mice, indicating that CNTF may influence affective behavior in females by regulation of neurotransmission.

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