Recombinant CCL2 (C-C motif chemokine ligand 2)，Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT with polyhistidine tag at the N-terminus.
Tag	His Tag (N-term)
Predicted MW	The protein has a calculated MW of 9.49 kDa. The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).
Purity	>95% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to chemoattract BaF3 cells transfected with human CCR2A. The ED₅₀ for this effect is <20 ng/mL.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	P13500.1
Synonyms	Monocyte Chemotactic Protein-1,MCP-1
Summary	C-C Motif Chemokine Ligand 2 (CCL2) is an 8.44 kDa cytokine with 76 amino acid residues and is also known as monocyte chemoattractant protein 1 (MCP1). The CCL2 secreted from monocytes, macrophages, and dendritic cells. Upon binding with CCR4, it regulates many biological functions, such as recruitments of monocytes, memory T cells, and dendritic cells, response to TNF-α and Interferon-γ, angiogenesis, cellular homeostasis, and cytoskeleton organization.

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