IL2 (NM_000586) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	Sf9
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human IL-2, the region of Ala21-Thr153, from gene Accession# NM_000586
Tag	Tag Free
Predicted MW	15.4 kDa
Concentration	lot specific
Purity	Purity is >95%, as determined by Coomassie stained SDS-PAGE.
Buffer	0.22 µm filtered protein solution in 30% Acetonitrile, 0.1% TFA (trifluoroacetic acid)
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.5 - 2.0 ng/ml, corresponding to a specific activity of 0.5 - 2.0x10^6 units/mg, determined by the dose dependent stimulation of CTLL2 cell proliferation.
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_000577
Locus ID	3558
UniProt ID	P60568
Refseq Size	822
Cytogenetics	4q27
Refseq ORF	459
Synonyms	IL-2; lymphokine; TCGF
Summary	This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Sep 2020]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus

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