IL1 alpha (IL1A) (NM_000575) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human IL-1alpha;, the region of Ser113-Ala271, from gene Accession# NM_000575
Tag	Tag Free
Predicted MW	18 Da
Concentration	lot specific
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Buffer	10 mM NaH2PO4, 150 mM NaCl, pH 7.2
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 5 - 15 pg/ml, corresponding to a specific activity of 0.6-2.0 x 10^7 units/mg, determined by the dose dependent stimulation of D10 cells proliferation.
Endotoxin	Less than 0.01 ng per ug protein as determined by the LAL method
Reference Data
RefSeq	NP_000566
Locus ID	3552
UniProt ID	P01583
Refseq Size	2943
Cytogenetics	2q14.1
Refseq ORF	813
Synonyms	IL-1 alpha; IL-1A; IL1; IL1-ALPHA; IL1F1
Summary	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Apoptosis, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, Prion diseases, Type I diabetes mellitus

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