GM CSF (CSF2) (NM_000758) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	Pichia
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Ala18-Glu144
Tag	Tag Free
Predicted MW	14.5 kDa
Concentration	lot specific
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from a 0.2 um filtered solution of 10mM TrisHCl, 4% Mannitol, 1% Sucrose, pH 8.5.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Stability	Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Bioactivity	ED50 is less than 0.2 ng/ml as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. Specific Activity of 5.0 x 106 IU/ mg.
Endotoxin	< 0.1 EU per µg protein as determined by LAL test
Reference Data
RefSeq	NP_000749
Locus ID	1437
UniProt ID	P04141
Cytogenetics	5q31.1
Synonyms	CSF; GMCSF
Summary	The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis. [provided by RefSeq, Aug 2020]
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway

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