Lcmt2 (NM_177846) Mouse Recombinant Protein
CAT#: TP515677
Purified recombinant protein of Mouse leucine carboxyl methyltransferase 2 (Lcmt2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR215677 representing NM_177846
Red=Cloning site Green=Tags(s) MGPRGRQRRAGTVQSTNDSSSLSKRSLAAHGYVRDPFAALLVPGPVRRTPLIHRGYYVRARAVRHCVRAF LELTSALPSRTRAQILSLGSGSDSLYFRLKAAGLLARAAVWEVDFPDVSRLKAERIEETPELRAQTGPFK IGDSASSLCFESADYRILGADLRELQRLGEALDGAGLDATSPTLLLAEAVLTYLEPSSATALIAWAAQRF PDALFVIYEQMQPGDAFGQIMLQHFQRLHSPLHGLELFPVVKAQRQRFLQAGWTACSALDLNEFYRRLLS AEERQRVETLEPFDEYEEWHLKCSHYFILAASRGDILSETPVFEPSEASFQIDPASPSGFLSARVVTSDH QHSSLKRYGHASALLSPGVIFSAGGFGEQEGRHCRVSRFHVLSRSCDSEWEGCQISTLGTEGQWDGRLYH TMTRLSDTRVLVLGGRLSPVSPASGALQLDLYKSKDNCSEGQNVTVTKAALEEGSVLSCWRHSTTEVYYQ NQRYLFVYGGRSVAEPVLSDCRFLHVETMAWVRIPVQGASPEGRHSHSACSWQGGALIAGGLGASEELLS SVLFLKPVSSGFLWESIDIQPSITPRYSHTAHVFNGKLLLVGGVWIHSSSVPGVTVICLTTGLSSEYQID TASVPWPLMLHNHSSALLPEEQQLLLIGGGGNCFSFGTYFNPHTVALDLSSLRSGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 75.3 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_808514 |
Locus ID | 329504 |
UniProt ID | Q8BYR1 |
Refseq Size | 3407 |
Cytogenetics | 2 E5 |
Refseq ORF | 2058 |
Synonyms | D330024M17; Tyw4 |
Summary | Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.[UniProtKB/Swiss-Prot Function] |
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