Gemin5 (NM_001166669) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR215628 protein sequence Red=Cloning site Green=Tags(s) MKPEPRTLPPSPNWYCSRCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGF TFSHHPGQYNLCATSSDDGTVKVWDVETKTVVTEHTLHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLN RNDSQHLFTEPRTIFCLTCSPHHENLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLSGED CLSISQEENSEEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPT VKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCSLKTEDGKQ LLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKN FWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGE GDRPSLTLYSCGGEGVVLQHNPWKLSGEAFDINKLVRDTNSIRYKLPVHTEISWKGDGKVLALGNEDGSI EIFQVPNLRLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPDSPITITE PYRTLSGHTAKITSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYS GADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPVKQDSSVGNEDESVKENS GPAENGLSDQDGEEEAQEPELPPSPVVCVEPVSCTDISSGFEKSKVTVSSKATSLKKEPPKEKPEALLKK RKARSMLPLSTSLDHRSKEELHRDCLVLATATHAKAELNEDVSADLEERFHLGLFTDRATLYRMMETEGK GHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKA ASYLLSIHKVYEAVELLKSNHLYREAIAVAKARLRPEDPVLKELYLSWGSILERDGHYAIAAKCYLGATS AYDAAKVLARKGDAASLRTAAELAAIAGEHELAASLALRCAQELLLVKNWVGAQEALGLHESLQGQRLVF CLLELLCRHLEEKQPLEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCQAALQKLQDVKYPS ATSNTPFRQLLLHVCHDLTLAMLSQQAAAWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRD KLGDLSPAMAAYKSLEAFCIYGQLYEVWWSLCGPGPESSVWVLSAESTVSDKQSKPEDSAGAEDMEQPPG PGPRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATTNGPSRDEPSRDE PSQEAERAPSQPPSPTEERNAPVSLPELTRRLTEANERIAEFPETVKAWPFPDVLECCLVLLHIGSQCPD AVDPEMQQQAQELLHKYGHTRAYRRHCQSRHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	166.5 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_001160141
Locus ID	216766
UniProt ID	Q8BX17, E9PUU4, Q3TR97
Refseq Size	6229
Cytogenetics	11 B1.3
Refseq ORF	4509
Synonyms	AA407055; AA407208; AI451603; BB194447; C330013N08
Summary	Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules (By similarity). Binds to the 3' UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (By similarity).[UniProtKB/Swiss-Prot Function]

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