Med25 (NM_029365) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR210426 protein sequence Red=Cloning site Green=Tags(s) MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVV FNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHR VCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQP ADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSA AQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTV APGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDAN TKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIM GNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGA QQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPS GPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQ SWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	78.1 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_083641
Locus ID	75613
UniProt ID	Q8VCB2
Refseq Size	2291
Cytogenetics	7 28.95 cM
Refseq ORF	2238
Synonyms	2610034E13Rik; 2610529E18Rik; ESTM2
Summary	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function]

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