Grhl3 (NM_001013756) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR209332 protein sequence Red=Cloning site Green=Tags(s) MSNELDFRSVRLLKNDPVSFQKFPYSNEDEAWKTYLENPLTAATKAMMRVNGDEESVAALSFLYDYYMGP KEKRILSSSTGGRNDQGKKFYHSMDYEPDLAPLESPTHLMKFLTENVSGSPDYTDQLKKNNLLGLEGVLP TPGKTNTVPPGPSKLEASSMDSYLLPASDIYDNGSLNSLFESIHGVPPTQRWQPDSTFKDDPQESLLFPD ILKTSPDPPCPEDYPGLKSDFEYTLGSPKAIHIKAGESPMAYLNKGQFYPVTLRTPAGGKGLALSSSKVK SVVMVVFDNDKVPVEQLRFWRHWHSRQPTAKQRVIDVADCKENFNTVQHIEEVAYNALSFVWNVNEEAKV FIGVNCLSTDFSSQKGVKGVPLNLQIDTYDCGAGTERLVHRAVCQIKIFCDKGAERKMRDDERKQFRRKV KCPDSSNNAGIKGCLLSGFRGNETTYLRPETDLETQPVLFIPNLHFSSLQRPGGVVPSAGHSSSDRLPLK RTCSPFAEEFEPLPSKQAKEDDLQRVLLYVRRETEEVFDALMLKTPDLKGLRNAISEKYGLPEENICKVY KKCKRGILVNMDNNIIQHYSNHVAFLLDMGELDGKIQIILKEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	67.8 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_001013778
Locus ID	230824
UniProt ID	Q5FWH3
Refseq Size	2798
Cytogenetics	4 67.76 cM
Refseq ORF	1809
Synonyms	AI561912; ct; Get1; Som; Tfcp2l4
Summary	Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes. Essential for epidermal differentiation and barrier formation at the end of embryogenesis with TGM3 as critical direct target (PubMed:21081122, PubMed:20654612, PubMed:25347468). Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events such as eyelid fusion and epidermal wound repair (PubMed:21081122). Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury (PubMed:25347468). Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (PubMed:14608380, PubMed:20654612). Also required for proper development of the oral periderm (PubMed:24360809). No genetic interaction with GRHL1, no functional cooperativity due to diverse target gene selectivity (PubMed:21081122).[UniProtKB/Swiss-Prot Function]

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