Mapk4 (NM_172632) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR209124 protein sequence Red=Cloning site Green=Tags(s) MAEKGDCIASVYGYDLGGRFIDFQPLGFGVNGLVLSATDSRACRKVAVKKIVLSDARSMKHALREIKIIR RLDHDNIVKVYEVLGPKGSDLQGELFKFSVAYIVQEYMETDLACLLEQGTLTEDHAKLFMYQLLRGLKYI HSANVLHRDLKPANIFISTEDLVLKIGDFGLARIVDQHYSHKGYLSEGLVTKWYRSPRLLLSPNNYTKAI DMWAAGCILAEMLTGKMLFAGAHELEQMQLILDTIPVVREEDKEELLRVMPSFVSSTWEVKRPLRKLLPD VNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSCPEDEPTSQHPFRIEDEIDDIVLMAASQSQLSNW DRYPVSLSSDLEWRPDRCQDASEVQRDPRAGSTPLAEDVQVDPRKDSQSSSERFLEQSHSSMERAFEADY GRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAASAPPRAAVAADPVSREDEPASLFLEIA QWVKSTQSGSERASPPPDAPEPRLSASPPGHPTPIDGGASPQFDLDVFISRALKLCTKPEDLPENKLGDL NGACISEHPGDLVQTEAFSKERW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	65.6 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_766220
Locus ID	225724
UniProt ID	Q6P5G0
Refseq Size	4660
Cytogenetics	18 E2
Refseq ORF	1752
Synonyms	A330097D03Rik; Erk3; p63Mapk; Prkm4
Summary	Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.[UniProtKB/Swiss-Prot Function]

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