dnaK / Hsp70 (full length) Escherichia coli Protein
CAT#: SA6024X
dnaK / Hsp70 (full length) e. coli protein, 0.5 mg
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CNY 12,210.00
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Specifications
Product Data | |
Species | Escherichia coli |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPT KHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMP QIEVTFDIDA DGILHVSAKD KNSGKEQKIT IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD HLLHSTRKQV EEAGDKLPAD DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME IAQQQHAQQQ TAGADASANN AKDDDVVDAE FEEVKDKK
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Predicted MW | 69 kDa |
Concentration | lot specific |
Purity | >95% by SDS-PAGE |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM DTT, 10% Glycerol |
Preparation | Liquid purified protein |
Protein Description | DnaK (amino acids 1-638) was amplified by PCR and cloned into an E. coli expression vector. DnaK 1-638 was overexpressed in E. coli and was purified to apparent homogeneity by using conventional column chromatography techniques. |
Storage | Store (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
Summary | DnaK, originally identified for its DNA replication by bacteriophage λ in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. |
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