PDGFB (PDGF-BB) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	24.3 kDa
Purity	>95% pure by SDS-PAGE and visualised by silver stain.
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: 50 mM Acetic Acid without stabilizer
Preparation	Lyophilized purified protein
Protein Description	Recombinant Human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Storage	Lyophilized Human PDGF-BB is stable for a few weeks at RT but best stored at -20°C. Reconsituted is best stored at -20°C to -70°C. Avoid repeated freezing and thawing.
Stability	Shelf life: One year from despatch.
Bioactivity	Biological: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb-c 3T3 cells is < 1 ng/ml. Specific: > 1 x 10e6 units/mg
Endotoxin	< 0.1 ng per ug of PDGF-BB
Reference Data
RefSeq	NP_002599
Locus ID	5155
UniProt ID	P01127
Cytogenetics	22q13.1
Synonyms	Platelet-derived growth factor subunit B, PDGF beta, PDGF B, PDGF-B, Platelet-derived growth factor beta, PDGF subunit B, Platelet-derived growth factor B chain, PDGF2, PDGF-2, c-Sis, SIS, Becaplermin
Summary	This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Protein Families	Druggable Genome
Protein Pathways	Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma

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