Thrombopoietin Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
Predicted MW	18.6 kDa
Purity	>98% pure by SDS-PAGE
Buffer	Presentation State: Purified State: Lyophilized (freeze-dried) without buffer and stabilizer
Preparation	Lyophilized (freeze-dried) without buffer and stabilizer
Protein Description	Thrombopoietin Human Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a Molecular Mass of 18608 Dalton which comprises the receptor binding domain of the Mpl-ligand protein.
Note	Centrifuge vials before opening!
Storage	Lyophilized TPO although stable at Room Temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human TPO should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Avoid repeated freeze-thaw cycles!
Stability	Shelf life: One year from despatch.
Bioactivity	Biological: The ED50 as determined by the dose-dependent stimulation of MO7e cells (with SCF) is 1,95 ng/ml, corresponding to a specific activity of 5 x 105 U/mg. Specific: 5 x 105 units/mg
Endotoxin	< 0.1 ng per µg (IEU/µg) of rh TPO
Reference Data
RefSeq	NP_000451
Locus ID	7066
UniProt ID	P40225
Cytogenetics	3q27.1
Synonyms	THPO, MGDF, C-mpl ligand
Summary	Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Hematopoietic cell lineage

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