Interleukin-4 / IL4 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	15 kDa
Purity	>98% pure by SDS-PAGE and HPLC analyses.
Buffer	Presentation State: Purified State: Lyophilized purified protein. Buffer System: PBS without stabilizer.
Preparation	Lyophilized purified protein.
Protein Description	Recombinant Human IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain having a molecular mass of 14.9 kDa and containing 129 amino acid residues.
Note	Centrifuge vial before opening!
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Recombinant Human IL-4 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is 0.1-0.3 ng/ml. For most in vitro applications, IL-4 exerts its biological activity in the concentration range of 0.1 to 10.0 ng/ml. Specific: 5 x 106 units/mg
Endotoxin	< 0.1 ng per µg (IEU/µg) of IL-4
Reference Data
RefSeq	NP_000580
Locus ID	3565
UniProt ID	P05112, D4HNR6
Cytogenetics	5q31.1
Synonyms	BCGF-1; BCGF1; BSF-1; BSF1; IL-4
Summary	The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway

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