VEGF-A (Isoform 164) Mouse Protein

Specifications

Product Data
Species	Mouse
Expression Host	Insect
Predicted MW	48 kDa
Purity	>95% > 95%, by SDS-PAGE and visualised by silver stain.
Buffer	Presentation State: Purified State: Lyophilized without stabilizer.
Preparation	Lyophilized without stabilizer.
Protein Description	Recombinant Murine VEGF164 is produced as a homodimer consisting of 164 amino acid residues.
Storage	Store VEGF164 Lyophilized at -20°C to -70°C. Reconstituted VEGF164 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Stability	Shelf life: One year from despatch.
Bioactivity	Biological: The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF164 has been determined to be in the range of 1-2 ng/ml. Specific: 1 x 10e6 units/mg
Endotoxin	< 0.1 ng/µg of VEGF
Reference Data
RefSeq	NP_001020421
Locus ID	22339
UniProt ID	Q00731
Cytogenetics	17 22.79 cM
Synonyms	VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Summary	This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.[provided by RefSeq, Nov 2015]

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