FGF basic / FGF2 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) AGSITTLPAL PEDGGSGAFP PGHFKDPKRL YCKNGGFFLR IHPDGRVDGV REKSDPHIKL QLQAEERGVV SIKGVCANRY LAMKEDGRLL ASKCVTDECF FFERLESNNY NTYRSRKYTS WYVALKRGQY KLGSKTGPGQ KAILFLPMSA KS
Predicted MW	16.5 kDa
Purity	>98% by SDS-PAGE and visualised by silver stain
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS Stabilizer: None
Preparation	Lyophilized purified protein
Protein Description	Recombinant Human Fibroblast Growth Factor-2 (basic). Result by N-terminal sequencing: AGSITTL
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: The ED50 for stimulation of cell proliferation in Human umbilical vein endothelial cells by Human FGF-2 (basic) has been determined to be in the range of 0.1-2 ng/ml.
Endotoxin	< 0.1 ng per µg of FGF-basic
Reference Data
RefSeq	NP_001997
Locus ID	2247
UniProt ID	P09038
Cytogenetics	4q28.1
Synonyms	FGFB, Heparin-binding growth factor 2, Fibroblast growth factor 2 (basic), BFGF, HBGF-2, HBGF2
Summary	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

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