Hepatitis B E Antigen / HBeAg Protein

Specifications

Product Data
Expression Host	E. coli
Concentration	lot specific
Purity	GS-4B Sepharose-Affinity chromatography.
Buffer	Presentation State: Purified State: Liquid purified fraction (>95% pure by 10% PAGE coomassie staining). Buffer System: 25 mM Tris-HCl, pH 8.0, 1.5 M Urea containing 50% glycerol without preservatives.
Preparation	Liquid purified fraction (>95% pure by 10% PAGE coomassie staining).
Applications	Suitable for use in ELISA and Western blot.
Protein Description	Hepatitis B Antigen 'e' epitope (HBe), Recombinant. Contains the HBe immunodominant region. Contains GST fusion partner. Immunoreactive with HBV positive sera.
Storage	Store the antigen at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid multiple freeze/thaw cycles.
Stability	Shelf life: six months from despatch.
Reference Data
Summary	Hepatitis B e-antigen (HBeAg) is a viral protein associated with HBV infections. Unlike the surface antigen, the e-antigen is found in the blood only when there are viruses also present. When the virus goes into “hiding,” the e-antigen will no longer be present in the blood. HBeAg is often used as a marker of ability to spread the virus to other people (infectivity). Measurement of e-antigen may also be used to monitor the effectiveness of HBV treatment; successful treatment will usually eliminate HBeAg from the blood and lead to development of antibodies against e-antigen (anti-HBe). There are some types (strains) of HBV that do not make e-antigen; these are especially common in the Middle East and Asia. In areas where these strains of HBV are common, testing for HBeAg is not very useful.
Protein Families	Suitable for use in ELISA and Western blot.

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