Vimentin Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	54 kDa
Concentration	1.0 mg/ml (after reconstitution)
Purity	>95% pure determined by SDS Gel Electrophoresis
Buffer	Presentation State: Purified State: Lyophilized protein Buffer System: Final solution contains 30 mM Tris/HCI pH 8, 9.5M Urea, 2 mM DTT, 2 mM EDTA, 10 mM Methylammonium Chloride
Preparation	Lyophilized protein
Applications	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (Phosphate Buffered Saline, e.g. Dulbecco's PBS).
Protein Description	Standard Recombinant Human Vimentin.
Note	Isoelectric Point: pI 5.3
Storage	Store at 2-8°C (lyophilized) or at -20°C (reconstituted). Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_003371
Locus ID	7431
UniProt ID	P08670
Cytogenetics	10p13
Synonyms	VIM
Summary	This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Protein Families	Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)&sub_category=Recombinant Proteins&families_filter=Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)">Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (Phosphate Buffered Saline, e.g. Dulbecco's PBS).

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