Interleukin-12 alpha / IL12A Human Protein

Specifications

Product Data
Species	Human
Expression Host	CHO
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) p35 Subunit: RNLPVA TPDPGMFPCL HHSQNLLRAV SNMLQKARQT LEFYPCTSEE IDHEDITKDK TSTVEACLPL ELTKNESCLN SRETSFITNG SCLASRKTSF MMALCLSSIY EDLKMYQVEF KTMNAKLLMD PKRQIFLDQN MLAVIDELMQ ALNFNSETVP QKSSLEEPDF YKTKIKLCIL LHAFRIRAVT IDRVMSYLNA S p40 Subunit: IWELKK DVYVVELDWY PDAPGEMVVL TCDTPEEDGI TWTLDQSSEV LGSGKTLTIQ VKEFGDAGQY TCHKGGEVLS HSLLLLHKKE DGIWSTDILK DQKEPKNKTF LRCEAKNYSG RFTCWWLTTI STDLTFSVKS SRGSSDPQGV TCGAATLSAE RVRGDNKEYE YSVECQEDSA CPAAEESLPIE VMVDAVHKL KYENYTSSFF IRDIIKPDPP KNLQLKPLKN SRQVEVSWEY PDTWSTPHSY FSLTFCVQVQ GKSKREKKDR VFTDKTSATV ICRKNASISV RAQDRYYSSS WSEWASVPCS
Predicted MW	75 kDa
Purity	>98% by SDS-PAGE and HPLC
Buffer	Presentation State: Purified State: Lyophilized purified protein Stabilizer: None
Preparation	Lyophilized purified protein
Protein Description	Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: The ED50 was determined by the induction of IFN-γ from NK cells co-stimulated with IL-18 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Endotoxin	< 0.1 ng per µg of IL-12
Reference Data
RefSeq	NP_000873
Locus ID	3592
UniProt ID	P29459
Cytogenetics	3q25.33
Synonyms	IL-12A, IL-12 subunit p35, NKSF1, CLMF p35
Summary	This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus

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