VEGF-A (VEGF164) Rat Protein

Specifications

Product Data
Species	Rat
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) APTTEGEQKA HEVVKFMDVY QRSYCRPIET LVDIFQEYPD EIEYIFKPSC VPLMRCAGCC NDEALECVPT SESNVTMQIM RIKPHQSQHI GEMSFLQHSR CECRPKKDRT KPEKCDKPRR Result by N-terminal sequencing: APTTEGEQKA H
Predicted MW	19.23 kDa
Purity	>95%
Buffer	Presentation State: Purified State: Lyophilized freeze dried protein Buffer System: PBS without stabilizer
Preparation	Lyophilized freeze dried protein
Protein Description	Recombinant Rat Vascular Endothelial Growth Factor 164
Storage	Lyophilized samples are stable for greater than six months at –20°C to -70°C. Reconstituted VEGF164 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Bioactivity	Biological: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 2-10 ng/ml.
Endotoxin	< 0.1 ng/µg of Rat VEGF164
Reference Data
RefSeq	NP_001103803
Locus ID	83785
UniProt ID	B5DEK7
Cytogenetics	9q12
Synonyms	VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Summary	This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

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