Cytokeratin 18 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	48,201
Concentration	lot specific
Purity	>95% pure (determined by SDS gelelectrophoresis)
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: 30 mM Tris/HCI pH 8, 9.5M Urea, 2 mM DTT, 2 mM EDTA, 10 mM Methylammonium Chloride. Restore with 175 µl distilled water (final volume 250µl)
Preparation	Lyophilized purified protein
Applications	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g.Dulbecco's PBS).
Protein Description	Recombinant Human keratin K18 Standard (formerly also designated Cytokeratin 18).
Note	Isoelectric Point: pI 5.7
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_000215
Locus ID	3875
UniProt ID	P05783
Cytogenetics	12q13.13
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Summary	KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Protein Families	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g.Dulbecco's PBS).
Protein Pathways	Pathogenic Escherichia coli infection

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