AKT1 / PKB (Substrate) Human Protein
CAT#: AR08011PU-N
AKT1 / PKB (Substrate) human protein, 0.5 mg
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CNY 5,080.00
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规格
Cited in 3 publications. |
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Specifications
Product Data | |
Species | Human |
Predicted MW | 817 Da |
Concentration | lot specific |
Purity | >98% pure by HPLC analysis. |
Buffer | Presentation State: Purified State: Liquid sterile filtered peptide |
Preparation | Liquid sterile filtered peptide |
Applications | Western Blot: 1/500-1/2,000. |
Protein Description | A specific substrate for Protein Kinase B alpha (PKB alpha, AKT; Km = 5 µM). This peptide is not phosphorylated by p70 S6 kinase or MAP kinase activated protein (MAPKAP) kinase-1. |
Storage | Store the antibody (ibn aliquots) at -70°C or below prior to opening. Avoid repeated freezing and thawing. |
Stability | Shelf life: 6 month from despatch. |
Reference Data | |
RefSeq | NP_001014431 |
Locus ID | 207 |
UniProt ID | P31749, B0LPE5 |
Cytogenetics | 14q32.33 |
Synonyms | AKT; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
Summary | This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020] |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
Protein Pathways | Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway |
Citations (3)
The use of this Proteins has been cited in the following citations: |
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Identification of AKT1/β-catenin mutations conferring cetuximab and chemotherapeutic drug resistance in colorectal cancer treatment
,null,
Oncology Letters
,PubMed ID 33574948
[AKT1]
|
Cytotoxic amphiphiles and phosphoinositides bind to two discrete sites on the Akt1 PH domain.
,null,
Biochemistry
,PubMed ID 24383815
[AKT1]
|
Discovery of a novel class of AKT pleckstrin homology (PH) domain inhibitors
,null,
,PubMed ID 18790745
[AKT1]
|
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