SARS-CoV-2 Spike-Membrane Recombinant Fusion Protein (Bioactivity Verified)

CAT#: TP701119

Purified recombinant fusion protein that includes the RBD of SARS-CoV-2 Spike protein (S-Protein) and Virion Surface Domain of Membrane Protein (M-protein) with C-terminal HIS-tag



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货期*
现货

规格
    • 50 ug

Cited in 1 publication.

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Specifications

Product Data
Species SARS-CoV-2
Expression Host HEK293T
Expression cDNA Clone or AA Sequence
MALWIDRMQL LSCIALSLAL VTNSAKGIYQ TSNFRVQPTE SIVRFPNITN LCPFGEVFNA
TRFASVYAWN RKRISNCVAD YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG
DEVRQIAPGQ TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF
ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS FELLHAPATV
CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLGGGS GGGSMADSNG TITVEELKKH
HHHHH
Tag C-HIS
Predicted MW 34 kDa
Concentration >0.05 µg/µL as determined by Bradford protein assay method.
Purity > 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer PBS, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity Binding of SARS-CoV-2 S-M Fusion Protein to Human ACE2: 2 ug/ml of SARS-CoV-2 Spike and Membrane viral fusion protein (gel insert) was immobilized on an ELISA plate and its ability to bind to Human ACE2 was measured. The EC50 was between 10-20 ng/ml.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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