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Osteopontin (SPP1) (NM_001040058) Human Over-expression Lysate
CAT＃: LY421887
Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 1
Size: 20 ug
View other "Osteopontin" proteins (13)
|
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CNY 3,080.00
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CNY 1,999.00
CNY 2,700.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 1 |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC204803 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 35.4 kDa |
| Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT) |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001035147 |
| Locus ID | 6696 |
| Cytogenetics | 4q22.1 |
| Protein Families | WB |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC400422 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC421887 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC424632 | SPP1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY400422 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 3 |
CNY 3,080.00 |
|
| LY424632 | Transient overexpression lysate of secreted phosphoprotein 1 (SPP1), transcript variant 2 |
CNY 3,080.00 |
|
| PH304803 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035147) |
CNY 14,250.00 |
|
| PH305118 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_000573) |
CNY 14,250.00 |
|
| PH310592 | SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035149) |
CNY 19,520.00 |
|
| TP304803 | Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, 20 µg |
CNY 2,900.00 |
|
| TP305118 | Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 2, 20 µg |
CNY 2,900.00 |
|
| TP310592 | Purified recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 3, with C-terminal MYC/DDK tag, secretory expressed in HEK293 cells, 20 µg |
CNY 2,900.00 |
|
| TP710213 | Purified protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, residues 17-314aa, secretory expressed with GP67 signal peptide, with C-terminal DDK tag, expressed in sf9, 20ug |
CNY 4,020.00 |
|
| TP720449 | Recombinant protein of human secreted phosphoprotein 1 (SPP1), transcript variant 2 |
CNY 3,140.00 |
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