GPAM (NM_020918.1) Human Over-expression Lysate

CAT#: LY412216

Transient overexpression lysate of human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM)



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CNY 4,140.00


货期*
4周

规格
    • 100 ug

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Specifications

Product Data
Description Transient overexpression lysate of human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205509
Tag C-MYC/DDK
Predicted MW 93.9 kDa
Components 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Avoid repeated freeze-thaw cycles. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Preparation HEK293T cells in 10-cm dishes were transiently transfected with PEI and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 72hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -80°C before shipping.
Reference Data
Locus ID 57678
Cytogenetics 10q25.2
Protein Families WB
Protein Pathways Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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