Hbb-bt (NM_008220) Mouse Over-expression Lysate
CAT#: LC727259
Hbb-b1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
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CNY 1,470.00
CNY 600.00
CNY 300.00
CNY 1,430.00
Specifications
Product Data | |
Description | Hbb-b1 HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone MR227259 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 15.7 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_032246 |
Locus ID | 101488143 |
Cytogenetics | 7 |
Protein Families | WB |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LY727259 | Transient overexpression lysate of Mouse hemoglobin, beta adult major chain (Hbb-b1) |
CNY 2,990.00 |
|
TP527259 | Purified recombinant protein of Mouse hemoglobin, beta adult major chain (Hbb-b1), full length, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug |
CNY 2,900.00 |