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CD47 (NM_001777) Human Over-expression Lysate
CAT＃: LC419754
CD47 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "CD47" proteins (9)
|
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CNY 900.00
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CNY 1,999.00
CNY 2,700.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | CD47 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC218813 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 35.21 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_001768 |
| Locus ID | 961 |
| Cytogenetics | 3q13.12 |
| Protein Families | WB |
| Protein Pathways | ECM-receptor interaction |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC404792 | CD47 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY404792 | Transient overexpression lysate of CD47 molecule (CD47), transcript variant 2 |
CNY 3,080.00 |
|
| LY419754 | Transient overexpression lysate of CD47 molecule (CD47), transcript variant 1 |
CNY 3,080.00 |
|
| TP700196 | Purified recombinant protein of Homo sapiens CD47 molecule (CD47), residues 19-141aa, with C-terminal Fc tag, expressed in HEK293 cells. |
CNY 6,650.00 |
|
| TP700197 | Purified recombinant protein of Homo sapiens CD47 molecule (CD47), residues 19-141aa, with C-terminal DDK/His tag, expressed in HEK293 cells. |
CNY 6,650.00 |
|
| TP710231 | Purified recombinant protein of Human CD47 molecule (CD47), transcript variant 1, residues 19-141aa, with C-terminal DDK tag, expressed in sf9, 20ug |
CNY 4,020.00 |
|
| TP720635 | Purified recombinant protein of Human CD47 molecule (CD47), transcript variant 2 |
CNY 3,140.00 |
|
| TP720959 | Purified recombinant protein of Human CD47 molecule (CD47), transcript variant 2 |
CNY 3,140.00 |
|
| TP723931 | Human CD47 Protein, mFc-His Tag |
CNY 5,876.00 |
