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Wilms Tumor Protein (WT1) (NM_024425) Human Over-expression Lysate
CAT＃: LC411289
WT1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "Wilms Tumor Protein" proteins (8)
|
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CNY 1,470.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | WT1 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC221847 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 54.4 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_077743 |
| Locus ID | 7490 |
| Cytogenetics | 11p13 |
| Protein Families | WB |
Citations (2)
| The use of this Proteins has been cited in the following citations: |
|---|
|
Depletion of dAKAP1–protein kinase A signaling islands from the outer mitochondrial membrane alters breast cancer cell metabolism and motility
,null,
The Journal of Biological Chemistry
,PubMed ID 30598507
[WT1]
|
|
Dichotomous roles for the orphan nuclear receptor NURR1 in breast cancer
,null,
BMC Cancer
,PubMed ID 23517088
[WT1]
|
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC424762 | WT1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
| LC434129 | WT1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY411289 | Transient overexpression lysate of Wilms tumor 1 (WT1), transcript variant C |
CNY 4,840.00 |
|
| LY424762 | Transient overexpression lysate of Wilms tumor 1 (WT1), transcript variant A |
CNY 4,840.00 |
|
| LY434129 | Transient overexpression lysate of Wilms tumor 1 (WT1), transcript variant F |
CNY 3,080.00 |
|
| TP760644 | Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant B, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug |
CNY 2,040.00 |
|
| TP760989 | Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant A, full length, with N-terminal HIS tag, expressed in E. coli, 50ug |
CNY 2,040.00 |
|
| TP762366 | Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant B, Met1-Ala78-GGGGS-Lys141-Gly229, with N-terminal His tag, expressed in E.coli, 50ug |
CNY 1,950.00 |
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