Heparanase 1 (HPSE) (NM_006665) Human Over-expression Lysate
CAT#: LC401992
HPSE HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "Heparanase 1" proteins (5)
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CNY 900.00
Cited in 3 publications. |
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
Product Data | |
Description | HPSE HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC208556 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 61.2 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_006656 |
Locus ID | 10855 |
Cytogenetics | 4q21.23 |
Protein Families | WB |
Protein Pathways | Glycosaminoglycan degradation, Metabolic pathways |
Citations (3)
The use of this Proteins has been cited in the following citations: |
---|
Heparanase regulates the M1 polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury.
,null,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
,PubMed ID 28970256
[HPSE]
|
Heparanase: A Potential New Factor Involved in the Renal Epithelial Mesenchymal Transition (EMT) Induced by Ischemia/Reperfusion (I/R) Injury
,null,
PLoS ONE
,PubMed ID 27467172
[HPSE]
|
Everolimus-induced epithelial to mesenchymal transition in immortalized human renal proximal tubular epithelial cells: key role of heparanase
,null,
Journal of Translational Medicine
,PubMed ID 24256696
[HPSE]
|
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC420636 | HPSE HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
LY401992 | Transient overexpression lysate of heparanase (HPSE), transcript variant 1 |
CNY 3,080.00 |
|
LY420636 | Transient overexpression lysate of heparanase (HPSE), transcript variant 2 |
CNY 4,840.00 |
|
TP760616 | Purified recombinant protein of Human heparanase (HPSE), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug |
CNY 2,040.00 |
|
TP762663 | Purified recombinant protein of Human heparanase (HPSE), transcript variant 2 |
CNY 2,480.00 |
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