Heparanase 1 (HPSE) (NM_006665) Human Tagged ORF Clone
CAT#: RC208556
HPSE (Myc-DDK-tagged)-Human heparanase (HPSE), transcript variant 1
ORF Plasmid: tGFP
"NM_006665" in other vectors (6)
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CNY 4,040.00
Cited in 5 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HPA; HPA1; HPR1; HPSE1; HSE1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208556 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCGCTCGAAGCCTGCGCTGCCGCCGCCGCTGATGCTGCTGCTCCTGGGGCCGCTGGGTCCCC TCTCCCCTGGCGCCCTGCCCCGACCTGCGCAAGCACAGGACGTCGTGGACCTGGACTTCTTCACCCAGGA GCCGCTGCACCTGGTGAGCCCCTCGTTCCTGTCCGTCACCATTGACGCCAACCTGGCCACGGACCCGCGG TTCCTCATCCTCCTGGGTTCTCCAAAGCTTCGTACCTTGGCCAGAGGCTTGTCTCCTGCGTACCTGAGGT TTGGTGGCACCAAGACAGACTTCCTAATTTTCGATCCCAAGAAGGAATCAACCTTTGAAGAGAGAAGTTA CTGGCAATCTCAAGTCAACCAGGATATTTGCAAATATGGATCCATCCCTCCTGATGTGGAGGAGAAGTTA CGGTTGGAATGGCCCTACCAGGAGCAATTGCTACTCCGAGAACACTACCAGAAAAAGTTCAAGAACAGCA CCTACTCAAGAAGCTCTGTAGATGTGCTATACACTTTTGCAAACTGCTCAGGACTGGACTTGATCTTTGG CCTAAATGCGTTATTAAGAACAGCAGATTTGCAGTGGAACAGTTCTAATGCTCAGTTGCTCCTGGACTAC TGCTCTTCCAAGGGGTATAACATTTCTTGGGAACTAGGCAATGAACCTAACAGTTTCCTTAAGAAGGCTG ATATTTTCATCAATGGGTCGCAGTTAGGAGAAGATTTTATTCAATTGCATAAACTTCTAAGAAAGTCCAC CTTCAAAAATGCAAAACTCTATGGTCCTGATGTTGGTCAGCCTCGAAGAAAGACGGCTAAGATGCTGAAG AGCTTCCTGAAGGCTGGTGGAGAAGTGATTGATTCAGTTACATGGCATCACTACTATTTGAATGGACGGA CTGCTACCAGGGAAGATTTTCTAAACCCTGATGTATTGGACATTTTTATTTCATCTGTGCAAAAAGTTTT CCAGGTGGTTGAGAGCACCAGGCCTGGCAAGAAGGTCTGGTTAGGAGAAACAAGCTCTGCATATGGAGGC GGAGCGCCCTTGCTATCCGACACCTTTGCAGCTGGCTTTATGTGGCTGGATAAATTGGGCCTGTCAGCCC GAATGGGAATAGAAGTGGTGATGAGGCAAGTATTCTTTGGAGCAGGAAACTACCATTTAGTGGATGAAAA CTTCGATCCTTTACCTGATTATTGGCTATCTCTTCTGTTCAAGAAATTGGTGGGCACCAAGGTGTTAATG GCAAGCGTGCAAGGTTCAAAGAGAAGGAAGCTTCGAGTATACCTTCATTGCACAAACACTGACAATCCAA GGTATAAAGAAGGAGATTTAACTCTGTATGCCATAAACCTCCATAATGTCACCAAGTACTTGCGGTTACC CTATCCTTTTTCTAACAAGCAAGTGGATAAATACCTTCTAAGACCTTTGGGACCTCATGGATTACTTTCC AAATCTGTCCAACTCAATGGTCTAACTCTAAAGATGGTGGATGATCAAACCTTGCCACCTTTAATGGAAA AACCTCTCCGGCCAGGAAGTTCACTGGGCTTGCCAGCTTTCTCATATAGTTTTTTTGTGATAAGAAATGC CAAAGTTGCTGCTTGCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208556 protein sequence
Red=Cloning site Green=Tags(s) MLLRSKPALPPPLMLLLLGPLGPLSPGALPRPAQAQDVVDLDFFTQEPLHLVSPSFLSVTIDANLATDPR FLILLGSPKLRTLARGLSPAYLRFGGTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKL RLEWPYQEQLLLREHYQKKFKNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDY CSSKGYNISWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTAKMLK SFLKAGGEVIDSVTWHHYYLNGRTATREDFLNPDVLDIFISSVQKVFQVVESTRPGKKVWLGETSSAYGG GAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVFFGAGNYHLVDENFDPLPDYWLSLLFKKLVGTKVLM ASVQGSKRRKLRVYLHCTNTDNPRYKEGDLTLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGPHGLLS KSVQLNGLTLKMVDDQTLPPLMEKPLRPGSSLGLPAFSYSFFVIRNAKVAACI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006665 |
ORF Size | 1629 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006665.2, NP_006656.1 |
RefSeq Size | 4668 bp |
RefSeq ORF | 1632 bp |
Locus ID | 10855 |
UniProt ID | Q9Y251 |
Domains | Glyco_hydro_79n |
Protein Families | Secreted Protein |
Protein Pathways | Glycosaminoglycan degradation, Metabolic pathways |
MW | 61.2 kDa |
Gene Summary | Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
Citations (5)
The use of this cDNA Clones has been cited in the following citations: |
---|
Ischemic stroke disrupts the endothelial glycocalyx through activation of proHPSE via acrolein exposure
,Ko, K;Suzuki, T;Ishikawa, R;Hattori, N;Ito, R;Umehara, K;Furihata, T;Dohmae, N;Linhardt, RJ;Igarashi, K;Toida, T;Higashi, K;,
J Biol Chem
,PubMed ID 33127645
[Heparanase 1]
|
Heparanase Activates Antithrombin through the Binding to Its Heparin Binding Site
,Bohdan, N;Espín, S;Águila, S;Teruel-Montoya, R;Vicente, V;Corral, J;Martínez-Martínez, I;,
PLoS ONE
,PubMed ID 27322195
[Heparanase 1]
|
Everolimus-induced epithelial to mesenchymal transition in immortalized human renal proximal tubular epithelial cells: key role of heparanase
,null,
Journal of Translational Medicine
,PubMed ID 24256696
[Heparanase 1]
|
Heparanase and Syndecan-1 Interplay Orchestrates Fibroblast Growth Factor-2-induced Epithelial-Mesenchymal Transition in Renal Tubular Cells
,null,
The Journal of Biological Chemistry
,PubMed ID 22102278
[Heparanase 1]
|
Regulation of heparanase by albumin and advanced glycation end products in proximal tubular cells.
,null,
Biochimica et biophysica acta
,PubMed ID 21600934
[Heparanase 1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208556L1 | Lenti ORF clone of Human heparanase (HPSE), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC208556L2 | Lenti ORF clone of Human heparanase (HPSE), transcript variant 1, mGFP tagged |
CNY 6,440.00 |
|
RC208556L3 | Lenti ORF clone of Human heparanase (HPSE), transcript variant 1, Myc-DDK-tagged |
CNY 6,440.00 |
|
RC208556L4 | Lenti ORF clone of Human heparanase (HPSE), transcript variant 1, mGFP tagged |
CNY 6,440.00 |
|
RG208556 | HPSE (tGFP-tagged) - Human heparanase (HPSE), transcript variant 1 |
CNY 5,640.00 |
|
SC111172 | HPSE (untagged)-Human heparanase (HPSE), transcript variant 1 |
CNY 4,048.00 |
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