MTHFD2 (NM_006636) Human Over-expression Lysate
CAT#: LC401984
MTHFD2 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "MTHFD2" proteins (6)
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CN¥ 900.00
Cited in 1 publication. |
Specifications
Product Data | |
Description | MTHFD2 HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC202143 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 37.9 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_006627 |
Locus ID | 10797 |
Cytogenetics | 2p13.1 |
Protein Families | WB |
Protein Pathways | Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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Suppression of MTHFD2 in MCF-7 Breast Cancer Cells Increases Glycolysis, Dependency on Exogenous Glycine, and Sensitivity to Folate Depletion.
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Journal of proteome research
,PubMed ID 27315223
[MTHFD2]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC421742 | MTHFD2 HEK293T cell transient overexpression lysate (as WB positive control) |
CN¥ 900.00 |
|
LY401984 | Transient overexpression lysate of methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CN¥ 3,080.00 |
|
LY421742 | Transient overexpression lysate of methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CN¥ 3,080.00 |
|
PH302143 | MTHFD2 MS Standard C13 and N15-labeled recombinant protein (NP_006627) |
CN¥ 19,520.00 |
|
TP302143 | Recombinant protein of human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), nuclear gene encoding mitochondrial protein, transcript variant 1, 20 µg |
CN¥ 2,900.00 |
|
TP760045 | Purified recombinant protein of Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), nuclear gene encoding mitochondrial protein, tra, residues 103-350aa, with N-terminal HIS tag, expressed in E.Coli, 50ug |
CN¥ 2,040.00 |