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p21 Ras (HRAS) (NM_005343) Human Over-expression Lysate
CAT＃: LC401645
HRAS HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "p21 Ras" proteins (10)
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CNY 900.00
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Specifications
| Product Data | |
| Description | HRAS HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC216409 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 21.1 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_005334 |
| Locus ID | 3265 |
| Cytogenetics | 11p15.5 |
| Protein Families | WB |
| Protein Pathways | Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Endocytosis, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway |
Documents
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC406123 | HRAS HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC427210 | HRAS HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC430451 | HRAS HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY401645 | Transient overexpression lysate of v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1 |
CNY 3,080.00 |
|
| LY406123 | Transient overexpression lysate of v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 2 |
CNY 3,080.00 |
|
| LY427210 | Transient overexpression lysate of v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 3 |
CNY 3,080.00 |
|
| PH316409 | HRAS MS Standard C13 and N15-labeled recombinant protein (NP_005334) |
CNY 19,520.00 |
|
| PH325202 | HRAS MS Standard C13 and N15-labeled recombinant protein (NP_001123914) |
CNY 19,520.00 |
|
| TP316409 | Recombinant protein of human v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1, 20 µg |
CNY 2,900.00 |
|
| TP325202 | Recombinant protein of human v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 3, 20 µg |
CNY 2,900.00 |
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