GFAP Bovine Protein

Specifications

Product Data
Description	GFAP bovine protein, 0.25 mg
Species	Bovine
Protein Source	Spinal Cord
Predicted MW	52 kDa
Concentration	lot specific
Purity	>98% (determined by SDS gelelectrophoresis).
Buffer	Presentation State: Purified State: Lyophilized Buffer System: Final Solution contains 10 mM Sodium Phosphate buffer pH 7.5, 6M Urea, 2 mM DTT, 1 mM EDTA, 10 mM Methylammonium Chloride
Reconstitution	Restore with distilled water. BA1011S: 80 µl (final volume 100 µl). BA1011 : 200 µl (final volume 250 µl).
Preparation	Lyophilized
Applications	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS).
Protein Description	Bovine Glial Filament Protein (GFP)
Note	Isoelectric Point: pI 5.4
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store the protein undiluted at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_776490
Locus ID	281189
Synonyms	Glial Fibrillary Acidic Protein
Summary	glial fibrillary acidic protein
Protein Families	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS).

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