Salivary alpha amylase (AMY1A) (NM_004038) Human Mass Spec Standard

Specifications

Product Data
Description	AMY1A MS Standard C13 and N15-labeled recombinant protein (NP_004029)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC217403
Predicted MW	57.8 kDa
Protein Sequence	Sequence Link (showhide) >Peptide sequence encoded by RC217403 Blue=ORF Red=Cloning site Green=Tag(s) MKLFWLLFTIGFCWAQYSSNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVAIHNP FRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGS RDFPAVPYSGWDFNDGKCKTGSGDIENYNDATQVRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVA GFRIDASKHMWPGDIKAILDKLHNLNSNWFPEGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAK LGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDARLYKMAVGFMLA HPYGFTRVMSSYRWPRYFENGKDVNDWVGPPNDNGVTKEVTINPDTTCGNDWVCEHRWRQIRNMVNFRN VVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWTFSLTLQTGLPAGTYCDVISGDKINGNCTGIKIY VSDDGKAHFSISNSAEDPFIAIHAESKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC217403 also available, TP317403
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_004029
RefSeq Size	1862
RefSeq ORF	1533
Synonyms	AMY1
Locus ID	276
Cytogenetics	1p21.1
Summary	Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Protein Families	ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Metabolic pathways, Starch and sucrose metabolism

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