CRYL1 (NM_015974) Human Mass Spec Standard

Specifications

Product Data
Description	CRYL1 MS Standard C13 and N15-labeled recombinant protein (NP_057058)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC212304
Predicted MW	35.2 kDa
Protein Sequence	Sequence Link (showhide) >RC212304 representing NM_015974 Red=Cloning site Green=Tags(s) MASSAAGCVVIVGSGVIGRSWAMLFASGGFQVKLYDIEQQQIRNALENIRKEMKLLEQAGSLKGSLSVEE QLSLISGCPNIQEAVEGAMHIQECVPEDLELKKKIFAQLDSIIDDRVILSSSTSCLMPSKLFAGLVHVKQ CIVAHPVNPPYYIPLVELVPHPETAPTTVDRTHALMKKIGQCPMRVQKEVAGFVLNRLQYAIISEAWRLV EEGIVSPSDLDLVMSEGLGMRYAFIGPLETMHLNAEGMLSYCDRYSEGIKHVLQTFGPIPEFSRATAEKV NQDMCMKVPDDPEHLAARRQWRDECLMRLAKLKSQVQPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_057058
RefSeq Size	1516
RefSeq ORF	957
Synonyms	GDH; gul3DH; HEL30; lambda-CRY
Locus ID	51084
Cytogenetics	13q12.11
Summary	The uronate cycle functions as an alternative glucose metabolic pathway, accounting for about 5% of daily glucose catabolism. The product of this gene catalyzes the dehydrogenation of L-gulonate into dehydro-L-gulonate in the uronate cycle. The enzyme requires NAD(H) as a coenzyme, and is inhibited by inorganic phosphate. A similar gene in the rabbit is thought to serve a structural role in the lens of the eye. [provided by RefSeq, Jul 2008]

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