CRYL1 (NM_015974) Human Tagged ORF Clone
CAT#: RC212304
CRYL1 (Myc-DDK-tagged)-Human crystallin, lambda 1 (CRYL1)
ORF Plasmid: tGFP
"NM_015974" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GDH; gul3DH; HEL30; lambda-CRY |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC212304 representing NM_015974
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCTCCGCGGCCGGCTGCGTGGTGATCGTTGGCAGTGGAGTCATTGGGCGAAGCTGGGCCATGC TGTTTGCCAGTGGAGGCTTCCAGGTGAAACTCTATGACATTGAGCAACAGCAGATAAGGAACGCCCTGGA AAACATCAGAAAGGAGATGAAGTTGCTGGAGCAGGCAGGTTCTCTGAAAGGCTCCCTGAGTGTGGAAGAG CAGCTGTCACTCATCAGTGGTTGTCCCAATATCCAAGAAGCAGTAGAGGGTGCCATGCACATTCAGGAAT GTGTTCCAGAAGATCTAGAACTGAAGAAGAAGATTTTTGCTCAGTTAGATTCCATCATTGATGATCGAGT GATCTTAAGCAGTTCCACTTCTTGTCTCATGCCTTCCAAGTTGTTTGCTGGCTTGGTCCATGTGAAGCAA TGCATCGTGGCTCATCCTGTGAATCCGCCATACTACATCCCGCTGGTTGAGCTGGTCCCCCACCCGGAGA CGGCCCCTACGACAGTGGACAGAACCCACGCCCTGATGAAGAAGATTGGACAGTGCCCCATGCGAGTCCA GAAGGAGGTGGCCGGCTTCGTTCTGAACCGCCTGCAATATGCAATCATCAGCGAGGCCTGGCGGCTAGTG GAGGAAGGAATCGTGTCTCCTAGTGACCTGGACCTTGTCATGTCAGAAGGGTTGGGCATGCGGTATGCAT TCATTGGACCCCTGGAAACCATGCATCTCAATGCAGAAGGTATGTTAAGCTACTGCGACAGATACAGCGA AGGCATAAAACATGTCCTACAGACTTTTGGACCCATTCCAGAGTTTTCCAGGGCCACTGCTGAGAAGGTT AACCAGGACATGTGCATGAAGGTCCCTGATGACCCGGAGCACTTAGCTGCCAGGAGGCAGTGGAGGGACG AGTGCCTCATGAGACTCGCCAAGTTGAAGAGTCAAGTGCAGCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212304 representing NM_015974
Red=Cloning site Green=Tags(s) MASSAAGCVVIVGSGVIGRSWAMLFASGGFQVKLYDIEQQQIRNALENIRKEMKLLEQAGSLKGSLSVEE QLSLISGCPNIQEAVEGAMHIQECVPEDLELKKKIFAQLDSIIDDRVILSSSTSCLMPSKLFAGLVHVKQ CIVAHPVNPPYYIPLVELVPHPETAPTTVDRTHALMKKIGQCPMRVQKEVAGFVLNRLQYAIISEAWRLV EEGIVSPSDLDLVMSEGLGMRYAFIGPLETMHLNAEGMLSYCDRYSEGIKHVLQTFGPIPEFSRATAEKV NQDMCMKVPDDPEHLAARRQWRDECLMRLAKLKSQVQPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015974 |
ORF Size | 957 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015974.3 |
RefSeq Size | 1516 bp |
RefSeq ORF | 960 bp |
Locus ID | 51084 |
UniProt ID | Q9Y2S2 |
Domains | 3HCDH, 3HCDH_N |
MW | 35.2 kDa |
Gene Summary | The uronate cycle functions as an alternative glucose metabolic pathway, accounting for about 5% of daily glucose catabolism. The product of this gene catalyzes the dehydrogenation of L-gulonate into dehydro-L-gulonate in the uronate cycle. The enzyme requires NAD(H) as a coenzyme, and is inhibited by inorganic phosphate. A similar gene in the rabbit is thought to serve a structural role in the lens of the eye. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212304L3 | Lenti ORF clone of Human crystallin, lambda 1 (CRYL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC212304L4 | Lenti ORF clone of Human crystallin, lambda 1 (CRYL1), mGFP tagged |
CNY 5,890.00 |
|
RG212304 | CRYL1 (tGFP-tagged) - Human crystallin, lambda 1 (CRYL1) |
CNY 4,370.00 |
|
SC317486 | CRYL1 (untagged)-Human crystallin, lambda 1 (CRYL1) |
CNY 6,270.00 |