MRCL3 (MYL12A) (NM_006471) Human Mass Spec Standard

Specifications

Product Data
Description	MYL12A MS Standard C13 and N15-labeled recombinant protein (NP_006462)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC207294
Predicted MW	19.8 kDa
Protein Sequence	Sequence Link (showhide) >RC207294 protein sequence Red=Cloning site Green=Tags(s) MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLD AMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDE LYREAPIDKKGNFNYIEFTRILKHGAKDKDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_006462
RefSeq Size	1243
RefSeq ORF	513
Synonyms	HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3; MYL2B
Locus ID	10627
Cytogenetics	18p11.31
Summary	This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014]
Protein Pathways	Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction

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