C14orf142 (GON7) (NM_032490) Human Mass Spec Standard

Specifications

Product Data
Description	C14orf142 MS Standard C13 and N15-labeled recombinant protein (NP_115879)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC206178
Predicted MW	10.9 kDa
Protein Sequence	Sequence Link (showhide) >RC206178 protein sequence Red=Cloning site Green=Tags(s) MELLGEYVGQEGKPQKLRVSCEAPGDGDPFQGLLSGVAQMKDMVTELFDPLVQGEVQHRVAAAPDEDLDG DDEDDAEDENNIDNRTNFDGPSAKRPKTPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_115879
RefSeq Size	1191
RefSeq ORF	300
Synonyms	C14orf142; PNAS-127
Locus ID	84520
Cytogenetics	14q32.12
Summary	Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. GON7 likely plays a supporting role to the catalytic subunit OSGEP in the complex.[UniProtKB/Swiss-Prot Function]

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