DPH3 (NM_206831) Human Mass Spec Standard

Specifications

Product Data
Description	DPH3 MS Standard C13 and N15-labeled recombinant protein (NP_996662)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC204203
Predicted MW	9.2 kDa
Protein Sequence	Sequence Link (showhide) >RC204203 protein sequence Red=Cloning site Green=Tags(s) MAVFHDEVEIEDFQYDEDSETYFYPCPCGDNFSITKEDLENGEDVATCPSCSLIIKVIYDKDQFVCGETV PAPSANKELVKC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_996662
RefSeq Size	4065
RefSeq ORF	246
Synonyms	DELGIP; DELGIP1; DESR1; DPH3A; KTI11; ZCSL2
Locus ID	285381
Cytogenetics	3p25.1
Summary	This gene encodes a CSL zinc finger-containing protein that is required for dipthamide biosynthesis. The encoded protein is necessary for the initial step in the modification of a histidine residue in elongation factor-2 to diphthamide. This modified residue is a target for ADP ribosylation by the bacterial toxins diphtheria toxin and Pseudomonas exotoxin A. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Feb 2009]

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