Twist (TWIST1) (NM_000474) Human Mass Spec Standard

Specifications

Product Data
Description	TWIST1 MS Standard C13 and N15-labeled recombinant protein (NP_000465)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC202920
Predicted MW	21 kDa
Protein Sequence	Sequence Link (showhide) >Peptide sequence encoded by RC202920 Blue=ORF Red=Cloning site Green=Tag(s) MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSP AQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTL PSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC202920 also available, TP302920
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_000465
RefSeq Size	1669
RefSeq ORF	606
Synonyms	ACS3; bHLHa38; BPES2; BPES3; CRS; CRS1; CSO; SCS; SWCOS; TWIST
Locus ID	7291
Cytogenetics	7p21.1
Summary	This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Jul 2020]
Protein Families	Druggable Genome

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